Rapid separation of mononuclear hodgkin from multinuclear reed-sternberg cells

Hodgkin's lymphoma

By : Narisorn Kongruttanachok, Yvon E Cayre, Hans Knecht, Sabine Mai

DOI :

10.1532/LH96.12023

PMID :

24694783

Abstract

We describe a method to isolate small mononucleated Hodgkin (H) cells from multinucleated Reed Sternberg (RS) cells of Hodgkin lymphoma using the ScreenCell filter device. This filtration-based approach lends itself to future clinical applications in that it enables the separation of H and RS cells from lymph node biopsies, bone marrow aspirates, pleural effusions, and blood, including the isolation of monoclonal Hodgkin precursor cells from the blood.

Keywords

Hodgkin; Reed Sternberg; cell separation; filtration device.

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