Liquid Biopsy in Rare Cancers: Lessons from Hemangiopericytoma


Hemangiopericytoma (HPT) is a rare mesenchymal tumor of fibroblastic type and for its rarity is poorly studied. The most common sites of metastatic disease in patients with intracranial HPT are the bone, liver, and lung, suggestive for an hematogenous dissemination; for this reason, we investigated, for the first time, the presence of circulating tumor cells (CTCs) in hemangiopericytoma patient by CellSearch® and SceenCell® devices. Peripheral blood samples were drawn and processed by CellSearch, an EpCAM-dependent device, and ScreenCell®, a device size based. We found nontypical CTCs by CellSearch system and the immunofluorescence analysis performed on CTCs isolate by ScreenCell demonstrated the presence of single CTCs and CTC clusters. The molecular characterization of single CTCs and CTC clusters, using antibodies directed against EpCAM, CD34, cytokeratins (8, 18, and 19), and CD45, showed a great heterogeneity in CTC clusters. We believe that the present study may open a new scenario in the rare tumors: the introduction of the liquid biopsy and the molecular characterization of circulating tumor cells could lead to personalized targeted treatments and also for rare tumors.PMID: 29707475 PMCID: PMC5863319 DOI: 10.1155/2018/9718585

Nicolazzo C1Colangelo L2Corsi A3Carpino G4Gradilone A1Sonato C2Raimondi C1Gaudio E5Gazzaniga P1Gianni W2.

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1Department of Molecular Medicine, Circulating Tumor Cells Unit, Sapienza University of Rome, Viale Regina Elena 324, 00161 Rome, Italy.2Policlinico Umerto I, II Division of Internal Medicine and Geriatrics, Sapienza University of Rome, Viale del Policlinico 155, 00161 Rome, Italy.3Department of Radiological, Oncological and Anatomopathological Sciences, Sapienza University of Rome, Viale Regina Elena 324, 00161 Rome, Italy.4Department of Movement, Human and Health Sciences, Division of Health Sciences, Foro Italico University of Rome, Piazza Lauro De Bosis 6, 00135 Rome, Italy.5Department of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences, Sapienza University of Rome, Via Alfonso Borelli 50, 00161 Rome, Italy.

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