A comparative analysis of cancer hotspot mutation profiles in circulating tumour cells, circulating tumour DNA and matched primary lung tumour
Maria Leung2, Maxim Freidin1, Dasha Freidina1, Sanjay Popat3, Andrew Nicholson2, Alexandra Rice2, Angeles Montero Fernandez2, Eric Lim1,2
1National Heart and Lung Institute, Imperial College London; 2Royal Brompton Hospital, London; 3Royal Marsden Hospital, London
The aim of our work is to report the concordances between CTCs and ctDNA versus the primary FFPE tumour mutations using a NGS hotspot panel.
“Our results suggest on a next generation sequencing platform that the global genetic variant profile between DNA extracted from CTC had good agreement with FFPE primary tumour tissue, and the agreement between ctDNA and FFPE was much poorer!” said consultant thoracic surgeon Dr Eric Lim from Royal Brompton Hospital, London.Download PDF